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Studies on the polyphenol metabolism of tissue cultures derived from the tea plant (Camellia sinensis L.)

机译：茶树（Camellia sinensis L.）组织培养物中多酚代谢的研究

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摘要

1. The growth characteristics on various media of solid and liquid suspension cultures derived from the stem of the tea plant are described; chlorophyll and anthocyanin synthesis occurred in the light. 2. Only the simplest catechins and leucoanthocyanins were present in callus tissue, although oligomeric and polymeric leucoanthocyanin fractions were also represented. Light caused an increase in all monomeric components analysed, but inhibited polymerization of the leucoanthocyanins. 3. The polyphenol oxidase activity of cultures was comparable with that of the apical regions of the intact plant, and was inversely correlated with growth rate. 4. Growth was stimulated by hormonal variation, and inhibited by high concentrations of sucrose and by high light-intensity; polyphenol concentrations were generally inversely correlated with growth rate. 5. From the inability of callus tissue and of cultured root apices to synthesize complex catechins, it is inferred that complex catechin formation in intact plants is associated with the process of cell vacuolation.
机译：1.描述了源自茶树茎的固态和液态悬浮培养物在各种培养基上的生长特性；叶绿素和花青素的合成在光照下发生。 2.愈伤组织中仅存在最简单的儿茶素和花青素，尽管也存在寡聚和聚合的花青素级分。光导致分析的所有单体成分增加，但抑制了花色苷的聚合。 3.培养物的多酚氧化酶活性与完整植物的顶端区域相当，并且与生长速率成反比。 4.荷尔蒙变化刺激生长，高浓度蔗糖和高光照强度抑制生长；多酚浓度通常与生长速度成反比。 5.从愈伤组织和培养的根尖无法合成复杂的儿茶素，可以推断出完整植物中复杂的儿茶素形成与细胞空泡化过程有关。

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  • 作者

    Forrest, G. I.;

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  • 年度 1969
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  • 正文语种 en
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